Journal: Cancer Cell International

Article Title: Immune subtyping for pancreatic cancer with implication in clinical outcomes and improving immunotherapy

doi: 10.1186/s12935-021-01824-z

Figure Lengend Snippet: Relation between TGM2 and tumor immunosuppression in PDAC. a IHC analysis of TGM2 with our tissue microarray ( n = 97). The scale bars were shown as indicated: 100 μm and 20 μm. b Survival analysis of TGM2 with tissue microarray data ( p = 0.015). c , d Distribution of high- and low-TGM2 group across the three immune subtypes showed as percentage and number. e, f Immune cell composition of high- and low-TGM2 groups. g Enrichment comparison of M2 type macrophages ( p = 0.014), Tregs ( p = 0.068), pro-B cells ( p = 0.00052) and memory B cells ( p = 3.7e−06) between high- and low-TGM2 groups. h Comparison of IMs expression between high- and low-TGM2 groups: row is for the immunomodulators and column is for the gene expression value. (CD274, p = 1.0e−14; CD276, p = 1.1e−10; CTLA4, p = 5.6e−07; CX3CL1, p = 1.6e−06; EDNRB, p = 3.8e−06; HAVCR2, p = 4.3e−10; LAG3, p = 0.0046; PDCD1, p = 0.0121; TGFB1, p = 1.2e−12; TIGIT, p = 6.5e−06; TLR4, p = 3.8e−08; VTCN1, p = 0.0585). i Survival analysis of CD276 expression in high-TGM2 group (top) and VTCN1 expression in low-TGM2 group (bottom)

Article Snippet: The microarray chip was stained with anti-TGM2 (15100-1-AP, Proteintech, 1:200).

Techniques: Microarray, Comparison, Expressing, Gene Expression

Journal: PLoS ONE

Article Title: Protein Microarray Analysis of Antibody Responses to Plasmodium falciparum in Western Kenyan Highland Sites with Differing Transmission Levels

doi: 10.1371/journal.pone.0082246

Figure Lengend Snippet: The z-scores for signal intensity of antibody binding by age-stratified sera from the hilltop and valley bottom sites were progressively summed (Y axis) and plotted against each of the 119 immunogenic polypeptides (X axis) recognized on the microarray. Lines in shades of green represent sera from the valley bottom; lines in shades of brown, from the hilltop. The number ( n ) of samples in each sera group is shown in the plot legend.

Article Snippet: A protein microarray of P. falciparum ( Pf ) reactive antigens (Antigen Discovery Inc., Irvine, CA) displaying 854 sequence-verified polypeptides printed as in vitro transcription translation (IVTT) reactions as described in Davies et al. [ ] was used.

Techniques: Binding Assay, Microarray

Journal: Transfusion

Article Title: Abstract Presentations from the AABB Annual Meeting San Diego, CA ctober 7‐10, 2017

doi: 10.1111/trf.14286

Figure Lengend Snippet:

Article Snippet: From December 2012 to June 2016, DNA extracted from 138 HbSS patients was tested by Human Erythrocyte Antigen (HEA) BeadChip DNA array (BioArray/Immucor) at the time of extraction; then subsequently by ID CORE XT (Progenika Biopharma/Grifols).

Techniques: DNA Array, Sequencing

Journal: Transfusion

Article Title: Abstract Presentations from the AABB Annual Meeting San Diego, CA ctober 7‐10, 2017

doi: 10.1111/trf.14286

Figure Lengend Snippet:

Article Snippet: From December 2012 to June 2016, DNA extracted from 138 HbSS patients was tested by Human Erythrocyte Antigen (HEA) BeadChip DNA array (BioArray/Immucor) at the time of extraction; then subsequently by ID CORE XT (Progenika Biopharma/Grifols).

Techniques:

Journal: Journal of proteomics

Article Title: Whole genome identification of C. trachomatis immunodominant antigens after genital tract infections and effect of antibiotic treatment of pigtailed macaques

doi: 10.1016/j.jprot.2014.05.009

Figure Lengend Snippet: Pigtailed macaques entered in the experiment C. trachomatis serovar used to infect, site of inoculation, antibiotic treatment and number of samples collected

Article Snippet: At the time the experiments were performed combination therapy with different agents was included. table ft1 table-wrap mode="anchored" t5 caption a7 # Pigtaile d macaqu es Inoculati on site # serum samples before inoculati on Chlamy dia serovar # of inoculatio ns # serum samples after inoculati on # Pigtaile d macaqu es Treatment # serum sampl es after tx 15 Fallopian tubes 6 Doxycyclin e: 2.2mg/kg/d ay orally, 10 days Doxycyclin e: 16 9 6 × 10 6 Serovar D (PO124) 3X 13 1 2.2mg/kg/d ay orally, 10 days + Triamcinolo ne: 0.2mg/kg each, 3 days 1 2 Placebo x 10 days Doxycyclin 9 2 5 × 10 6 Serovar E 2X 3 2 e: 2.2mg/kg/d 3 (MTW47 7) ay orally, 10 days Doxycyclin e: 1 2.2mg/kg/d ay orally, 10 days Doxycyclin 1 4 6 × 10 6 Serovar E 2X 6 e: 2.2mg/kg/d ay orally, (MTW47 7) 2 10 days + Triamcinolo ne: 0.2mg/kg each, 3 days 4 1 Placebo x 10 days 0 5 × 10 4 Serovar 4 Placebo x 14 days 7 10 Cervix 7 E (MTW47 7) 5X 10 3 Placebo x 14 days 0 3 5 × 10 4 Serovar E (MTW47 7) 1X 8 3 Azithromyci n: 14mg/kg each, 5 days 0 Open in a separate window Pigtailed macaques entered in the experiment C. trachomatis serovar used to infect, site of inoculation, antibiotic treatment and number of samples collected Production of C. trachomatis proteome microarray chips The C. trachomatis protein microarray chips were prepared following a three steps process: 1) PCR amplification of the 894 open reading frames (ORF); 2) in vivo recombination cloning, and 3) in vitro transcription/translation followed by microarrays chip printing (Antigen Discovery, Inc., Irvine, CA).

Techniques:

Journal: Journal of proteomics

Article Title: Whole genome identification of C. trachomatis immunodominant antigens after genital tract infections and effect of antibiotic treatment of pigtailed macaques

doi: 10.1016/j.jprot.2014.05.009

Figure Lengend Snippet: Number of monkeys (% +) that gave positive signals with the 20 immunodominant antigens following infection with C. trachomatis

Article Snippet: At the time the experiments were performed combination therapy with different agents was included. table ft1 table-wrap mode="anchored" t5 caption a7 # Pigtaile d macaqu es Inoculati on site # serum samples before inoculati on Chlamy dia serovar # of inoculatio ns # serum samples after inoculati on # Pigtaile d macaqu es Treatment # serum sampl es after tx 15 Fallopian tubes 6 Doxycyclin e: 2.2mg/kg/d ay orally, 10 days Doxycyclin e: 16 9 6 × 10 6 Serovar D (PO124) 3X 13 1 2.2mg/kg/d ay orally, 10 days + Triamcinolo ne: 0.2mg/kg each, 3 days 1 2 Placebo x 10 days Doxycyclin 9 2 5 × 10 6 Serovar E 2X 3 2 e: 2.2mg/kg/d 3 (MTW47 7) ay orally, 10 days Doxycyclin e: 1 2.2mg/kg/d ay orally, 10 days Doxycyclin 1 4 6 × 10 6 Serovar E 2X 6 e: 2.2mg/kg/d ay orally, (MTW47 7) 2 10 days + Triamcinolo ne: 0.2mg/kg each, 3 days 4 1 Placebo x 10 days 0 5 × 10 4 Serovar 4 Placebo x 14 days 7 10 Cervix 7 E (MTW47 7) 5X 10 3 Placebo x 14 days 0 3 5 × 10 4 Serovar E (MTW47 7) 1X 8 3 Azithromyci n: 14mg/kg each, 5 days 0 Open in a separate window Pigtailed macaques entered in the experiment C. trachomatis serovar used to infect, site of inoculation, antibiotic treatment and number of samples collected Production of C. trachomatis proteome microarray chips The C. trachomatis protein microarray chips were prepared following a three steps process: 1) PCR amplification of the 894 open reading frames (ORF); 2) in vivo recombination cloning, and 3) in vitro transcription/translation followed by microarrays chip printing (Antigen Discovery, Inc., Irvine, CA).

Techniques: Infection, Membrane, Translocation Assay

Journal: Journal of proteomics

Article Title: Whole genome identification of C. trachomatis immunodominant antigens after genital tract infections and effect of antibiotic treatment of pigtailed macaques

doi: 10.1016/j.jprot.2014.05.009

Figure Lengend Snippet: Antibody responses in monkeys to the 20 immunodominant C. trachomatis antigens following antibiotic/placebo treatment

Article Snippet: At the time the experiments were performed combination therapy with different agents was included. table ft1 table-wrap mode="anchored" t5 caption a7 # Pigtaile d macaqu es Inoculati on site # serum samples before inoculati on Chlamy dia serovar # of inoculatio ns # serum samples after inoculati on # Pigtaile d macaqu es Treatment # serum sampl es after tx 15 Fallopian tubes 6 Doxycyclin e: 2.2mg/kg/d ay orally, 10 days Doxycyclin e: 16 9 6 × 10 6 Serovar D (PO124) 3X 13 1 2.2mg/kg/d ay orally, 10 days + Triamcinolo ne: 0.2mg/kg each, 3 days 1 2 Placebo x 10 days Doxycyclin 9 2 5 × 10 6 Serovar E 2X 3 2 e: 2.2mg/kg/d 3 (MTW47 7) ay orally, 10 days Doxycyclin e: 1 2.2mg/kg/d ay orally, 10 days Doxycyclin 1 4 6 × 10 6 Serovar E 2X 6 e: 2.2mg/kg/d ay orally, (MTW47 7) 2 10 days + Triamcinolo ne: 0.2mg/kg each, 3 days 4 1 Placebo x 10 days 0 5 × 10 4 Serovar 4 Placebo x 14 days 7 10 Cervix 7 E (MTW47 7) 5X 10 3 Placebo x 14 days 0 3 5 × 10 4 Serovar E (MTW47 7) 1X 8 3 Azithromyci n: 14mg/kg each, 5 days 0 Open in a separate window Pigtailed macaques entered in the experiment C. trachomatis serovar used to infect, site of inoculation, antibiotic treatment and number of samples collected Production of C. trachomatis proteome microarray chips The C. trachomatis protein microarray chips were prepared following a three steps process: 1) PCR amplification of the 894 open reading frames (ORF); 2) in vivo recombination cloning, and 3) in vitro transcription/translation followed by microarrays chip printing (Antigen Discovery, Inc., Irvine, CA).

Techniques:

Journal: Journal of proteomics

Article Title: Whole genome identification of C. trachomatis immunodominant antigens after genital tract infections and effect of antibiotic treatment of pigtailed macaques

doi: 10.1016/j.jprot.2014.05.009

Figure Lengend Snippet: The distribution of the predicted roles of the 20-immunodominant antigens is compared to the whole C. trachomatis serovar D and to all the reactive chlamydial antigens using the JCVI cellular role categories.

Article Snippet: At the time the experiments were performed combination therapy with different agents was included. table ft1 table-wrap mode="anchored" t5 caption a7 # Pigtaile d macaqu es Inoculati on site # serum samples before inoculati on Chlamy dia serovar # of inoculatio ns # serum samples after inoculati on # Pigtaile d macaqu es Treatment # serum sampl es after tx 15 Fallopian tubes 6 Doxycyclin e: 2.2mg/kg/d ay orally, 10 days Doxycyclin e: 16 9 6 × 10 6 Serovar D (PO124) 3X 13 1 2.2mg/kg/d ay orally, 10 days + Triamcinolo ne: 0.2mg/kg each, 3 days 1 2 Placebo x 10 days Doxycyclin 9 2 5 × 10 6 Serovar E 2X 3 2 e: 2.2mg/kg/d 3 (MTW47 7) ay orally, 10 days Doxycyclin e: 1 2.2mg/kg/d ay orally, 10 days Doxycyclin 1 4 6 × 10 6 Serovar E 2X 6 e: 2.2mg/kg/d ay orally, (MTW47 7) 2 10 days + Triamcinolo ne: 0.2mg/kg each, 3 days 4 1 Placebo x 10 days 0 5 × 10 4 Serovar 4 Placebo x 14 days 7 10 Cervix 7 E (MTW47 7) 5X 10 3 Placebo x 14 days 0 3 5 × 10 4 Serovar E (MTW47 7) 1X 8 3 Azithromyci n: 14mg/kg each, 5 days 0 Open in a separate window Pigtailed macaques entered in the experiment C. trachomatis serovar used to infect, site of inoculation, antibiotic treatment and number of samples collected Production of C. trachomatis proteome microarray chips The C. trachomatis protein microarray chips were prepared following a three steps process: 1) PCR amplification of the 894 open reading frames (ORF); 2) in vivo recombination cloning, and 3) in vitro transcription/translation followed by microarrays chip printing (Antigen Discovery, Inc., Irvine, CA).

Techniques: